Open AccessSTUDY THE ANTIMICROBIAL AND ANTIADHESIVE ACTIVITY OF PURIFIED BIOSURFACTANT PRODUCED FROM LACTOBACILLUS PLANTARUM AGAINST PATHOGENIC BACTERIA
Author(s) -
Aleaa abdul hussein jameel,
N. H. Haider
Publication year - 2021
Publication title -
iraqi journal of agricultural science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.232
H-Index - 7
eISSN - 2410-0862
pISSN - 0075-0530
DOI - 10.36103/ijas.v52i5.1457
Subject(s) - chemistry , antibacterial activity , chromatography , staphylococcus aureus , thin layer chromatography , bacteria , column chromatography , silica gel , pathogenic bacteria , microbiology and biotechnology , antimicrobial , chloroform , lactobacillus plantarum , lactic acid , organic chemistry , biology , genetics
This study was aimed to purification the biosurfactant that produced from Lactobacillus plantarum isolated from clinical samples of iraqi healthy women by column chromatography through silica gel column (3.5 × 30 cm) using solvent system (chloroform : methanol, 2:1) and characterization the purified product by Thin layer chromatography (TLC), Fourier Transform Infrared Red spectroscopy (FTIR) and Gas chromatography (GC) mass then evaluated its antibacterial and anti-adhesive activity. The results shown the higher emulsification activity (E24%= 63) and lower the surface tension to 23 mN/m in synthetic MSM, while the natural media gave (E24% =71) and lower surface tension to 19 mN/m. The using TLC technique indicated presence of lipopeptide in the biosurfactant with Rf value = 0.82. Antibacterial and antiadhesion activities were evaluated against some pathogenic bacteria, including Pseudomonas aureginosa and Staphylococcus aureus. The results showed a higher inhibitory effect of biosurfactant at concentration 200 mg/ml on S. aureus and P. aeruginosa with the inhibition zone 27 mm and 33 mm respectively in BCDFTM media. While purified biosurfactant with concentration 200 mg/ml produced in MSM media had an effect on S. aureus and P. aeruginosa with the inhibition zone 21 mm, and 18 mm respectively. The anti-adhesion activity of purified biosurfactant against p.aeruginosa ranging from (34.70 ± 5.256) to (11.7 ± 9.7) produced from natural media BCDFTM and was higher than the anti-adhesion activity of purified biosurfactant against the same pathogen ranging from (46.95 ± 7.37) to (20.15 ± 2.805) produced in MSM.