Open AccessROLE OF PLANT GROWTH REGULATORS IN CALLUS INITIATION AND PLANTLETS REPRODUCING OF TWO ALFALFA CULTIVARS
Author(s) -
Hamza Ali
Publication year - 2017
Publication title -
iraqi journal of agricultural science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.232
H-Index - 7
eISSN - 2410-0862
pISSN - 0075-0530
DOI - 10.36103/ijas.v48i3.389
Subject(s) - callus , cultivar , growth regulator , biology , horticulture , embryo , botany , kinetin , dry weight , plant growth , murashige and skoog medium , tissue culture , in vitro , biochemistry , microbiology and biotechnology
An experiment was conducted at the central lab of Graduate studies - College of Agriculture, University of Baghdad during 2015-2016. The objective was to study the effect of growth regulator on inducing callus from embryos of two alfalfa cultivars (PAC-78001 and local) variety by tissue culture technique using factorial experiments according to CRD. Seeds sterilized by NaOCl at 4.5% for 15 minutes, then, cultured on MS media free of growth regulators to embryo induced. The embryos were cultured on MS media at different concentrations of 2,4-D (0, 1, 2, 3 and 4) mg Li-1. Induced callus from embryos was cultured on MS media supplementing with Kin. at 0, 0.25, 0.50, 0.75 and 1 mg Li-1 to determine the best concentration. The results showed that best concentration of 2,4-D and Kin were 2 and 0.5 mg L-1 respectively which gave highest callus fresh weight and callus dry weight (204.65 and 21.50) mg Li-1, respectively. BA at 2 mg Li-1 gave the highest plantlets reproducing from callus amounted 5.5 vegetative growth tube-1.