Brassinosterioids Stimulate Nitrogen Metabolism of Pigeon Pea Plants under Water Deficit Conditions

This study was aimed to find the effects of 28-epibrassinolide (28-EBL) and HBL on pigeon pea seedlings subjected to drought stress, either alone and supplemented with 28-EBL and HBL treatments. Supplementation of EBL (2µM) and HBL (2µM) alone also exhibited the significant improvement in nodule number, nodule fresh weight, nodule dry weight, nodule Leghemoglobin content, root nodule nitrogenase (N2ase)under stress free conditions but under Drought stress conditions EBL (2µM) and HBL (2µM) exhibited the significant improvement in nodule number, nodule fresh weight ,nodule dry weight, nodule leghemoglobin content, root nodule nitrogenase (N2ase) while as control and other concentration (0.5,1 µM) didn’t performed up to level. Effect of Brassinosteroids increase the root nitrate reductase (NR), root nitrite reductase (NiR) activity, root nitrate (NO3-) content, root nitrite (NO2-) content, GS enzymes activities, under stress free conditions, under drought stress conditions this activity is ultimately low. Brassinosteroids at all concentration exhibited positive correlation with significantly raise in the root ammonium (NH4+) content and GOGAT enzymes activities. The present study shows that Pigeon pea plants under water stress, stimulate nitrogen metabolism effecting enzymes associated with it. But different concentration of 28-epibassinolide (0.5,1,2µM) and HBL were applied to the crop under drought stress- and stress-free conditions. Exogenous application of EBL and HBL promotes the Nitrogen Metabolism as plotted in the Graphs Further research is required for the detailed analysis.