Open AccessKinetic of 4-Chlorophenols Biodegradation by Arthrobacter Chlorophenolicus A6
Publication year - 2019
Publication title -
international journal of innovative technology and exploring engineering
Language(s) - English
Resource type - Journals
ISSN - 2278-3075
DOI - 10.35940/ijitee.l1070.10812s19
Subject(s) - biodegradation , arthrobacter , laboratory flask , substrate (aquarium) , chemistry , environmental chemistry , degradation (telecommunications) , microorganism , reaction rate constant , pollutant , bacterial growth , chromatography , bacteria , kinetics , food science , nuclear chemistry , organic chemistry , enzyme , biology , ecology , telecommunications , genetics , physics , quantum mechanics , computer science
Chlorophenols are listed as priority pollutants both by European community and US EPA. Biodegradation of pchlorophenol (4-CP) was investigated in batch shake flasks by Arthrobacter chlorophenolicus A6 at initial 4-CP concentrations between 25 to 350 mgl-1. The rate of 4-CP removal decreased with increasing initial 4-CP concentrations due to toxic effects on the microorganisms. The growth and biodegradation kinetic of the culture was evaluated. The batch growth profile of the A chlorophenolicus A6 followed substrate inhibition kinetics with the estimated biokinetic parameters of Ksi = 272 mgl-1, Ks = 65 mgl-1 for 4-CP respectively. High inhibition constant (KSI = 272 mg -l) with a KSI/KS ratio of 4.18 indicates superior 4-CP biodegradation potential of the A chlorophenolicus A6. The maximum rate of 4-CP degradation has been achieved at an optimum substrate concentration of Smax = (KSKSI)1/2 = (65×272)1/2 = 133 mgl−1.