Open AccessUsing Oleaginous Yeast in Spent Tea for Determination of Lipid Accumulation Capacity
Author(s) -
Mithun Sakthivel,
Mr. M. Deepak,
Mr. Pravin Kumar,
Mr. S Jaisurya
Publication year - 2020
Publication title -
international journal of innovative technology and exploring engineering
Language(s) - English
Resource type - Journals
ISSN - 2278-3075
DOI - 10.35940/ijitee.g5268.059720
Subject(s) - yarrowia , biomass (ecology) , food science , yeast , chemistry , fossil fuel , biodiesel , biofuel , pulp and paper industry , environmental science , microbiology and biotechnology , biology , organic chemistry , biochemistry , catalysis , agronomy , engineering
Alternative energy becomes the most chief mover of economic growth and plays an important role in this warming world. The whole world comes across the depletion of fossil fuels and has the degradation of the environment. Due to the depletion of fossil fuels, demand is also considerable increasing in demand and rapid increase in the petroleum prices. So, there is a need in search for other alternatives to fossil fuels. Biodiesel is considering being new alternatives to explore our dependency on oil imports that are help in protecting the environment towards the sustainable development. In this study, oleanginous yeast such as Lipomyces starkeyi, Metschnikowia pulcherrima, and Yarrowia lipolytica was cultured where spent tea wastes were used as a substrate. Depolymerization techniques like acid and alkaline treatments were carried out on the spent tea waste substrate. The initial qualitative and quantitative analysis of glucose were carried out by UV spectroscopy gave a maximum result on alkaline treatment. Hence alkaline treatment was selected for culturing and the various oleaginous yeasts were inoculated in the alkali depolymerized samples. After growth of 7 days, biomass was separated from media and was found to be 10.5 g/l, 10 g/l, 8.75 g/l for Y. lipolytica, L. starkeyi and M. pulcherrima. Lipids were isolated from biomass by Bligh and dryer method. Lipid confirmation was sorted out using FTIR.