Effect of High Dose Amlodipine to Nrf2 Protein on SH-SY5Y Cell Culture That Induced with Glucose 50 mM

Diabetes Mellitus is a chronic metabolic disease characteristic by the presence of chronic hyperglycemia that glucose levels > 200 mg/dL. Protein Nrf2 is a transcription factor protein for antioxidant genes that serves to capture reactive oxygen species (ROS) which increases in the condition of diabetes mellitus. To investigate the effect of the administration of amlodipine on protein expression of Nrf2 in SH-SY5Y neuron cell culture that induced by glucose 50 mM. This research were used the method of pure experimental analysis in the laboratory used cell cultures of the SH-SY5Y neurons. Cell culture SH-SY5Y neurons were induced with glucose 50 mM for 6 days. Then administered amlodipine with a dose of 5 µM for 30 minutes. Then expression of Nrf2 proteins observed with immunofluorescence. Independent t-test and Pearson statistical analyses were conducted to determine the influence and relationship of a 5 μM amlodipine on protein expression Nrf2. In the group of administering amlodipine 5 µM and without amlodipine on the cell culture of the SH-SY5Y neurons that induced of chronic hyperglycemia (glucose 50 mM) result of independent t-test obtained that there is no meaningful difference (P = 0.464). Pearson correlation test result in get a correlation coefficient value of 0.223. There is an increased expression of the protein Nrf2 after the administration of the dose of amlodipine 5 μM although not significant.