Open AccessApplication of Plackett Burman Design for Antioxidant Extraction from Actinodaphne sesquipedalis leaves
Author(s) -
Siti Fatihah Balqis Binti Jamaludin,
Siti Salwa Binti Abd Gani,
Uswatun Hasanah Binti Zaidin,
Paiman Bawon
Publication year - 2019
Publication title -
international journal of engineering and advanced technology
Language(s) - English
Resource type - Journals
ISSN - 2249-8958
DOI - 10.35940/ijeat.f1008.0986s319
Subject(s) - extraction (chemistry) , dpph , solvent , chemistry , ethanol , traditional medicine , box–behnken design , chromatography , botany , antioxidant , response surface methodology , biology , biochemistry , medicine
Actinodaphne sesquipedalis Hook. F. Var. Glabra (Kochummen), also known as “Medang payung” by the Malay people, belongs to the Lauraceae family. In this study, a Plackett-Burman design was used to evaluate the significant extraction parameters in achieving maximum DPPH radical scavenging activity from ethanol leaves extract of A. sesquipedalis. Microwave-assisted extraction technique was employed using aqueous ethanol. The independent parameters were microwave power level (30–60 W), feed-to-solvent ratio (1:30 g/ml), irradiation time (30-90 s) and ethanol concentration (20–80%). Amongst the examined parameters, ethanol concentration, microwave power level, and irradiation/extraction time were significant, whereas, feed-to-solvent ratio was insignificant. Therefore, antioxidants from the ethanolic extraction leaves of A. sesquipedalis using microwave technique are significantly affected by ethanol concentration, irradiation time and microwave power.