Open AccessCoelomic Fluid of Earthworm, Eudrilus Eugeniae, Inhibits the Growth of Fungal Hyphae, in Vitro
Author(s) -
Chandran Rajesh,
Rajamanikkam Kamaraj,
Ganapathy Nadana Raja Vadivu,
Karuppaiah Palanichelvam
Publication year - 2019
Publication title -
international journal of engineering and advanced technology
Language(s) - English
Resource type - Journals
ISSN - 2249-8958
DOI - 10.35940/ijeat.a1146.1291s419
Subject(s) - earthworm , hypha , fusarium oxysporum , microbiology and biotechnology , incubation , biology , rhizoctonia solani , coelom , aspergillus flavus , fungus , chemistry , botany , biochemistry , anatomy , ecology
Knowledge on interactions between earthworms and microbes at molecular level has been obscure. Mechanism by which earthworms defend themselves against different microorganisms in the soil is largely unknown. Coelomic fluid from earthworm species has been shown to have antimicrobial activity. In this study we report that coelomic fluid isolated from Eudrilus eugeniae has antifungal activity against four different phytopathogens such as Rhizoctonia solani, Fusarium oxysporum, Aspergillus flavus and Verticillium dahlia. Incubation of coelomic fluid with fungal hyphae of all these four strains for 12 hours revealed that the growth of hyphae was greatly inhibited. Light microscopy studies confirmed that the hyphae growth was greatly reduced by incubation of coelomic fluid. Analysis of the fluid in the microscope showed the presence of different types of coelomocytes in the mucous fluid. Other molecules and compounds in the coelomic fluid might also have played role in antifungal activity.