Open AccessINVESTIGATION OF PHYSICAL AND CHEMICAL PROPERTIES OF POTATO LEAFROLL VIRUS ISOLATES IN CULTURES OF MAMMALIAN CELLS
Author(s) -
T. Bova,
Ю. В. Волкова,
L. M. Reshotko
Publication year - 2014
Publication title -
sìlʹsʹkogospodarsʹka mìkrobìologìâ
Language(s) - English
Resource type - Journals
ISSN - 1997-3004
DOI - 10.35868/1997-3004.19.83-88
Subject(s) - virus , divalent , biology , dissolution , virology , chemistry , microbiology and biotechnology , organic chemistry
The paper presents the research data of physical and chemical properties of potato leaf rolling virus isolates in the PEKV and BHK-21 cell cultures. It was shown that in mammalian cell cultures the isolates of potatoes leaf rolling virus were resistant to the action of lipid dissolving agents, cultural media with pH from 4.0 to 10.0 and were thermoresistant at 50 °C in the absence of divalent ions of magnesium. The virus inactivation temperature point along with the optimum temperature for its reproduction were established.