Open AccessOPTIMIZATION OF CULTURAL MEDIA FOR JOINT CULTIVATION OF BRADYRHIZOBIUM JAPONICUM AND AZOSPIRILLUM BRASILENSE
Author(s) -
С. Ф. Козар
Publication year - 2014
Publication title -
sìlʹsʹkogospodarsʹka mìkrobìologìâ
Language(s) - English
Resource type - Journals
ISSN - 1997-3004
DOI - 10.35868/1997-3004.19.27-32
Subject(s) - bradyrhizobium japonicum , azospirillum brasilense , microbial inoculant , microorganism , bacteria , strain (injury) , yeast , symbiosis , biology , inoculation , food science , horticulture , rhizobiaceae , biochemistry , genetics , anatomy
The paper presents the results of studies on the optimization of culture media for joint cultivation of Bradyrhizobium japonicum and Azospirillum brasilense bacteria at periodic passages. It was established that cultivation of studied microorganisms in a mixed culture the number of nodule bacteria vary from 0.76 to 6.72 billion cells/cm3, depending on the concentration of medium components. It was shown that the effect of molasses, fodder yeast and phosphates on growth activity of B. japonicum significantly depends not only on the amount of chemical components in the media, but also on the strain of A. brasilense, used for joint cultivation. Based on the results of multivariate experiment an optimized media for joint cultivation of studied microorganisms was proposed.