Open AccessRAPID DIAGNOSTIC OF FISH FLAVOBACTERIOSIS USING POLYMERASE CHAIN REACTION
Author(s) -
Yu. Rud
Publication year - 2014
Publication title -
sìlʹsʹkogospodarsʹka mìkrobìologìâ
Language(s) - English
Resource type - Journals
ISSN - 1997-3004
DOI - 10.35868/1997-3004.18.132-145
Subject(s) - polymerase chain reaction , flavobacterium , 16s ribosomal rna , biology , fish <actinopterygii> , microbiology and biotechnology , phylogenetic tree , rainbow trout , gene , genetics , bacteria , fishery , pseudomonas
The method of rapid identification of fish flavobacteriosis using polymerase chain reaction (PCR) was developed. Specifity and efficiency of the selected oligonucleotide primers was confirmed. Nine Flavobacterium strains were isolated from clinically healthy and infected with Flavobacteriosis rainbow trout Onchorhynchus mykiss. Flavobacterium columnare strain was identified using the developed rapid diagnosis method. Phylogenetic analysis had indicated the kinship of isolated strains to Flavobacterium genera. Neither F. psychrophylum or F. branchiophilum strains were identified in the isolated samples. The species identification capacity using the 16S rRNA gene restriction analysis was shown. The developed PCR method can be used for rapid identification of different fish flavobacteriosis forms in fish farms in Ukraine.