Open AccessNEW INACTIVATED VACCINE AGAINST ENZOOTIC ENCEPHALOMYELITIS OF SWINES
Author(s) -
S. V. Derevianko,
Vladislav Soroka,
T. O. Bowa
Publication year - 2013
Publication title -
sìlʹsʹkogospodarsʹka mìkrobìologìâ
Language(s) - English
Resource type - Journals
ISSN - 1997-3004
DOI - 10.35868/1997-3004.17.170-184
Subject(s) - enzootic , virology , vaccination , epizootic , titer , antibody , outbreak , biology , medicine , virus , immunology
A specific inactivated vaccine for prevention of enzootic encephalomyelitis (Teschen disease) of swines was created. The vaccine is produced on the basis of «Dniprovskiy-34» strain of Porcine teschovirus-1 isolated in the Ukraine and adjuvant of ASRIAP. During vaccines production the original method of teshoviruses inactivation was used. A single vaccine dose of 2 cubic cm promotes specific swines immunity to Teschen disease remaining for 11 months. Protective antibody levels were observed on the 7th day after vaccination. The maximum level of virus neutralizing antibodies in the blood of vaccinated animals on 60th day have reached 1:8192. Geometric mean titer of antibodies is 1:512, which protects the vaccinated piglets even at intracerebral introduction of 10 lethal doses of control epizootic highly virulent strain of Teschen disease of swines «Chernihivskiy-2372». A high level of innovative product design was confirmed by commission tests conducted in cooperation with State Scientific Control Institute of Biotechnology and Microorganism Strains and protected with three patents of Ukraine for inventions and useful models. A normative product documentation (Technical Requirements of Ukraine, instructions for production and control and product insert) was created. Performed feasibility study of the «Teshovak» vaccine allows recommending it into the production and introduction into the practices of veterinary medicine.