Open AccessTHE BIOLOGICAL PROPERTIES OF SOYBEAN NODULE BACTERIA WITH THE DIFFERENT SPEED OF GROWTH
Author(s) -
D.V. Krutуlo
Publication year - 2010
Publication title -
sìlʹsʹkogospodarsʹka mìkrobìologìâ
Language(s) - English
Resource type - Journals
ISSN - 1997-3004
DOI - 10.35868/1997-3004.10.50-64
Subject(s) - bradyrhizobium japonicum , bradyrhizobium , biology , 16s ribosomal rna , rhamnose , strain (injury) , microbiology and biotechnology , bacteria , symbiosis , rhizobiaceae , polysaccharide , genetics , biochemistry , anatomy
The phenotypical and genotypic properties of soybean microsymbionts with the different speed of growth were studied. It was established that the strains with the intensive growth were specific to the host-plant, but had differed by their serological, chemotaxonomical and symbiotic properties. The investigated intensive-growing strains were combined into the one serogroup by their antigenic content. The similarity of fatty acids spectrum was observed for the strains with the intensive growth Bradyrhizobium sp. КВ1-1, slow-growing strains B. japonicum 634b and B. japonicum КС2-3. The quantitative and qualitative differences in monosaccharides contents of EPS of the studied strains were revealed. Presence of xylose (2,7 %) and increased contents of rhamnose (18,4 %) were shown to be different in EPS of strain Bradyrhizobium sp. КВ1- 1 and EPS of strains B. japonicum КС2-3 and B. japonicum 634b. Analysis of the sequences of the 16S rRNA genes allowed attributing the strain with the intensive growth to the Bradyrhizobium genus. Genes consistency analysis of 16S rRNA had allowed to refer the intensivegrowing strains to the Bradyrhizobium genus. It was shown that studied intensive-growing strains had formed the nonspecific symbiosis with cow pea.