Open AccessFringe- and speckle-free holographic patterned illumination using time-multiplexed temporal focusing
Author(s) -
Tomohiro Ishikawa,
Keisuke Isobe,
Kenta Inazawa,
Takayuki Michikawa,
Kamiki,
Atsushi Miyawaki,
Fumihiko Kannari,
Katsumi Midorikawa
Publication year - 2022
Publication title -
applied physics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.911
H-Index - 94
eISSN - 1882-0786
pISSN - 1882-0778
DOI - 10.35848/1882-0786/ac5dec
Subject(s) - holography , speckle pattern , optics , multiplexing , materials science , interference (communication) , resolution (logic) , electronic speckle pattern interferometry , physics , computer science , artificial intelligence , telecommunications , channel (broadcasting)
Multi-photon holographic patterned illumination using temporal focusing (TF) pulses provides 10 μ m multi-spots with an axial confinement of 5 μ m, enabling parallel photo-stimulation of multi-target cells in three dimensions. However, interference fringes and speckles degrade the uniformity of the spots, causing spatio-temporal fluctuations in photoactivation. Here, we demonstrate fringe- and speckle-free holographic patterned illumination using multifocal time-multiplexed (TM) TF pulses even when the multi-spots overlap spatially. The uniform patterned illumination enabled the expansion of the field-of-view of TM-TF microscopy by more than fivefold and enhanced the axial resolution to 1.3 μ m.