Fringe- and speckle-free holographic patterned illumination using time-multiplexed temporal focusing | Zendy

Tomohiro Ishikawa | Zendy; Keisuke Isobe | Zendy; Kenta Inazawa | Zendy; Takayuki Michikawa | Zendy; Kamiki | Zendy; Atsushi Miyawaki | Zendy; Fumihiko Kannari | Zendy; Katsumi Midorikawa | Zendy

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Fringe- and speckle-free holographic patterned illumination using time-multiplexed temporal focusing

Author(s) -

Tomohiro Ishikawa,

Keisuke Isobe,

Kenta Inazawa,

Takayuki Michikawa,

Kamiki,

Atsushi Miyawaki,

Fumihiko Kannari,

Katsumi Midorikawa

Publication year - 2022

Publication title -

applied physics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.911

H-Index - 94

eISSN - 1882-0786

pISSN - 1882-0778

DOI - 10.35848/1882-0786/ac5dec

Subject(s) - holography , speckle pattern , optics , multiplexing , interference (communication) , materials science , electronic speckle pattern interferometry , resolution (logic) , multiple exposure , temporal resolution , physics , computer science , artificial intelligence , telecommunications , channel (broadcasting)

Multi-photon holographic patterned illumination using temporal focusing (TF) pulses provides 10 μ m multi-spots with an axial confinement of 5 μ m, enabling parallel photo-stimulation of multi-target cells in three dimensions. However, interference fringes and speckles degrade the uniformity of the spots, causing spatio-temporal fluctuations in photoactivation. Here, we demonstrate fringe- and speckle-free holographic patterned illumination using multifocal time-multiplexed (TM) TF pulses even when the multi-spots overlap spatially. The uniform patterned illumination enabled the expansion of the field-of-view of TM-TF microscopy by more than fivefold and enhanced the axial resolution to 1.3 μ m.

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