Open AccessOptimization of a method for the determination of a mustard gas biomarker in human blood plasma by liquid chromatography–mass spectrometry
Publication year - 2017
Publication title -
vestnik vojsk rhb zaŝity
Language(s) - English
Resource type - Journals
ISSN - 2587-5728
DOI - 10.35825/2587-5728-2017-1-3-7-17
Subject(s) - chromatography , chemistry , detection limit , adduct , mass spectrometry , human blood , gas chromatography , tandem mass spectrometry , blood plasma , tripeptide , blood proteins , human plasma , high performance liquid chromatography , gas chromatography–mass spectrometry , biochemistry , organic chemistry , peptide , physiology , biology
A method for the determination of a mustard gas biomarker (an S-hydroxyethylthioethyl adduct with albumin) in blood plasma was optimized with the use of HPLC with tandem mass-spectrometricdetection. This method is based on the hydrolysis of this adduct by the proteinase K enzyme with the formation of the following stable tripeptide with cysteine, proline, and phenylalanine: S-HETE-Cys-Pro-Phe. The detection limit of mustard gas in human blood plasma was 2 ng/ml. The approach proposed was tested in the analysis of human blood plasma samples by the standard addition technique and also within the framework of the first official biomedical test carried out by theOrganization for the Prohibition of Chemical Weapons (OPCW) in 2016, and it exhibited a good accuracy, reproducibility, and specificity of determination.