Open AccessOligoclonality and subpopulation structure of bone marrow T-cells in patients with aplastic anaemia
Author(s) -
Anastasia Abramova,
Irina Galtseva,
Е. А. Михайлова,
Nikolay Kapranov,
Ю. О. Давыдова,
Zalina Fidarova,
В В Троицкая,
Е Н Паровичникова,
В Г Савченко
Publication year - 2020
Publication title -
gematologiâ i transfuziologiâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.126
H-Index - 5
eISSN - 2411-3042
pISSN - 0234-5730
DOI - 10.35754/0234-5730-2020-65-4-417-430
Subject(s) - cytotoxic t cell , immunology , cd8 , bone marrow , t cell , biology , immune system , t cell receptor , haematopoiesis , cytokine , interleukin 21 , stem cell , microbiology and biotechnology , biochemistry , in vitro
. The main pathogenetic mechanism of the development of aplastic anemia (AA) is a violation of the immune regulation of hematopoiesis. Aim : to study of the subpopulation composition of T-cells and the repertoire of the T-cell receptor in AA patients. Patients and Methods . The study included AA patients ( n = 40) without prior immunosuppressive therapy in 2018–2020. The T-cell subpopulation structure and T-cell receptor Vβ-family (TCR-Vβ) oligoclonality were studied in samples of bone marrow using ow cytometry. Results . We report characteristic properties of T-cell subpopulations of bone marrow in all AA patients: elevated counts of cytotoxic T-cells, effector CD4 + and CD8 + cells, CD4 + memory cells, which may suggest a long-term antigenic stimulation with subsequent activation of these cell subpopulations resulting in hyperexpression of pro-inammatory cytokines. Diminishing of naive CD4 + and CD8 + cells, regulatory and double negative T-cells may indicate a relaxing control of cytokine-producing T-cells. A relationship has been established between the AA severity and counts of effector, regulatory, double negative and PD-1 positive T-cells. A highest count of potentially cytokine-producing T-cells and lowest count of cells involved in T-cell activity regulation were observed in very severe AA patients. Studies of the TCR-Vβ repertoire revealed oligoclonal expansion in the cytotoxic T-cell subpopulation. Conclusion . Enrichment in selected Vβ families suggests autoreactive T-cell clonality and attests to the immune nature of AA. A dynamic TCR-Vβ repertoire assay may be recommended in the disease monitoring. Flow cytometry helps identify valuable biomarkers for T-cell clone monitoring in AA and a better assessment of the disease progression.