Open AccessBiofi lm production among Candida spp. causing invasive candidiasis in patients with hematological malignancies and without hematological malignancies
Author(s) -
А О Мальчикова,
Г А Клясова
Publication year - 2020
Publication title -
gematologiâ i transfuziologiâ
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.126
H-Index - 5
eISSN - 2411-3042
pISSN - 0234-5730
DOI - 10.35754/0234-5730-2020-65-3-281-290
Subject(s) - candida krusei , corpus albicans , candida parapsilosis , candida albicans , candida glabrata , microbiology and biotechnology , medicine , biology , candida tropicalis , gastroenterology
. Biofi lm-forming ability among Candida spp. on indwelling medical devices may have a negative infl uence on the outcome of invasive candidiasis in various groups of patients. Aim. The objective of this study was to evaluate the biofi lm-forming ability among Candida spp. isolated from clinical specimens in patients with hematological malignancies and patients without hematological malignancies. Materials and methods. Biofi lm production among Candida spp. was studied using XTT (Sigma-Aldrich, USA) reduction assay. Candida spp. were classifi ed as biofi lm-forming, having optical density equal to and more than 0.1, and non-biofi lmforming with optical density less than 0.1. Results. A total of 428 Candida spp. (C. albicans n = 192, C. parapsilosis n = 121, C. krusei n = 40, C. tropicalis n = 38, C. glabrata n = 37) were evaluated (172 from hematological patients, 256 from non-hematological patients, 361 from blood culture, 67 from other sterile specimens). Biofi lm-forming ability was detected among 179 (41.8%) Candida spp. with the same rate in hematological patients and non-hematological patients (41.9 % and 41.8 %, respectively). Biofi lm production predominated among non-C. albicans (52.5 %) compared to C. albicans (28.6 %, p = 0.001). Biofi lm production prevailed among C. tropicalis (89.5 %) and C. krusei (75 %) compared to C. parapsilosis (41.3 %), C. albicans (28.6 %), and C. glabrata (27 %, respectively, p < 0.05). Biofi lm-forming ability among C. tropicalis and C. krusei dominated in both groups of patients. Biofi lm production among C. albicans prevailed in non-hematological patients compared to hematological patients (34.1% vs 18.2%, p = 0.03). There were no differences in biofi lm production among Candida spp. isolated from blood culture (42.9%) and other sterile specimens (35.8%, p = 0.3). Conclusion. Biofi lm-forming ability varied among the Candida spp. and prevailed among C. tropicalis and C. krusei. Biofi lm production among Candida spp. was detected with the same rate in hematological and non-hematological patients.