Open AccessANTIOXIDANT AND ANTIGOUT ACTIVITY OF ULTRASONIC EXTRACT OF UNRIPE MUSA BALBISIANA COLLA PULP
Author(s) -
Erna Styani,
Candra Irawan,
Maman Sukiman,
Imalia Dwi Putri,
Andita Utami,
Avisani Dewanta
Publication year - 2022
Publication title -
xi'nan jiaotong daxue xuebao
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.308
H-Index - 21
ISSN - 0258-2724
DOI - 10.35741/issn.0258-2724.57.1.30
Subject(s) - dpph , antioxidant , chemistry , uric acid , pulp (tooth) , food science , extraction (chemistry) , xanthine oxidase , biochemistry , chromatography , enzyme , medicine , pathology
Ultrasonic-Assisted Extraction (UAE) technology is an effective method for the extraction of chemical constituents from plant materials. The antioxidant activity found in Musa balbisiana Colla may be related to antigout activity, where there is a role for antioxidants in the inhibition of xanthine oxidase activity. The extraction of secondary metabolites from Musa balbisiana Colla using the UAE method has never been reported; in addition, antioxidant testing using the FRAP method and uric acid test have never been carried out. This research aims to gain ultrasonic extract from the unripe Musa balbisiana Colla pulp using the UAE method and obtain information about secondary metabolites and their bioactivity as antioxidant and antigout agents. Ethanolic extract was obtained by the UAE method, total phenolic content was determined by the Folin–Ciocalteu method, antioxidant activity was measured using DPPH and FRAP method, then the uric acid level was measured using uric acid test kit. The results showed that the antioxidant activity test using the DPPH and FRAP methods gave IC50 values of 411.59 ± 0.1336 mg/L and 98.03 ± 0.2926 mg/L, respectively. The total phenolic content value of 823.95 ± 3.9806 mg GAE/g ultrasonic extract is thought to have a role in high antioxidant activity. Furthermore, ultrasonic extract with a concentration of 50 mg/L has activity in reducing uric acid levels by around 10%. It can be concluded that the ultrasonic extract produced by the UAE has potential as a source of antioxidant and antigout agents.