Open AccessTumor Necrosis Factor Alpha SNP Variant in Promoter Region G308a, cause Preeclampsia during Pregnancy in Pakistani Women, A Case Control Study
Author(s) -
Azizah Ziauddin,
Saeed Ahmad,
Asad Iqbal,
Abdul Majid Khan,
Shahid Mahmood
Publication year - 2020
Publication title -
journal of bioresource management
Language(s) - English
Resource type - Journals
ISSN - 2309-3854
DOI - 10.35691/jbm.0202.0154
Subject(s) - preeclampsia , single nucleotide polymorphism , genotyping , pregnancy , medicine , snp , genotype , snp genotyping , genetic predisposition , gastroenterology , immunology , obstetrics , disease , biology , genetics , gene
Preeclampsia (PE) is a very common critical condition during pregnancy. As PE is a high-risk condition during pregnancy, occurring in 25% of all pregnancies, worldwide. In women with PE there is an increase in hypertension and albuminuria. Elevated blood pressure can be life-threatening after 20th week of pregnancy. Single nucleotide variation in gene sequence can be disease causing, among these pathogenic SNPs, a variant in TNF-α, G308A is analyzed in many studies as a causative variant to cause preeclampsia. In this case control study fifty patients and fifty healthy individuals were enrolled for analysis of TNF-α promoter region SNP G308A from Jinnah hospital, Lahore, Pakistan. The genotyping of TNF-α (G308A) rs1800629 polymorphisms was performed by PCR-RFLP method. Data analysis was performed by using SNPStats, statistical tool. The mean age of all patients and controls were calculated, 24.4 ± 6.6 and 25.1 ± 5.3 years, respectively. The frequency of G308A polymorphism was more prevalent in the case group, in association with control group (p<0.001). There was a significant correlation between inflammation promoting genotypes of TNF-α and PE. It can be warily concluded that: TNF- α (G-308A) polymorphism can be reflected as a marker of predisposition to preeclampsia in our population.