Open AccessPeroxidase activity of hemoglobin and heme destruction in the presence of hydrogen peroxide and CT-DNA
Publication year - 2020
Publication title -
glasnik hemičara i tehnologa bosne i hercegovine
Language(s) - English
Resource type - Journals
eISSN - 2232-7266
pISSN - 0367-4444
DOI - 10.35666/ghtbh.2020.55.04
Subject(s) - hydrogen peroxide , peroxidase , heme , chemistry , hemoglobin , hypochlorous acid , myoglobin , cytochrome c peroxidase , biochemistry , peroxide , reactive oxygen species , lactoperoxidase , oxidative stress , enzyme , organic chemistry
The aim of this study was to investigate the peroxidase activity of Hb with different concentrations of hydrogen peroxide and compare it with hypochlorous acid effect on Hb. Hypochlorous acid at higher concentrations decomposed Hb and heme, releasing fee iron ion from the metal center. High concentrations of hydrogen peroxide switched the peroxidase activity of Hb towards the partial Hb and heme destruction. Heme alone was degraded showing that the Hb conformation and protein environment protects Hb from the distraction in the presence of highly increased hydrogen peroxide concentration that occurs as a result of oxidative stress. In the presence of CT-DNA acted inhibition of the peroxidase activity of Hb was observed signaling inhibited hydrogen peroxide consumption.