Open AccessAnalysis of the complete blood count in male Wistar rats exposed to different doses of orally administered sodium selenite
Author(s) -
А. В. Синдирева,
O. A. Zayko,
В. В. Асташов,
Inna Borodina,
Elizaveta Guseva,
Polina Zaytseva
Publication year - 2022
Publication title -
archiv euromedica
Language(s) - English
Resource type - Journals
eISSN - 2199-885X
pISSN - 2193-3863
DOI - 10.35630/2199-885x/2022/12/2.4
Subject(s) - selenium , lymphocytopenia , anemia , medicine , white blood cell , chemistry , endocrinology , lymphocyte , organic chemistry
Aims: To compare the structure of the formula and morphological characteristics of the blood cells after an acute experiment in laboratory rats with oral administration of sodium selenite at a concentration of selenium (Se) 0.5, 1 and 2 maximum permissible concentration (MPC). Methods: Four groups of Wistar rats weighing 260–300 g (a control group and three experimental ones: Se group of 0.5 MPC, 1 MPC and 2 MPC) were given sodium selenite in the respective concentrations for 5 days (except for the control group). After the rats were removed from the experiment, blood was taken from the caudal artery and a biochemical blood test was performed and the data obtained were evaluated and compared with the norm. Results: Changes in the cell composition of rat blood were determined by the dose of selenium. There were no changes in the "Se 0.5 MPC" group compared to the control group. In the Se 1 MPC group, the main difference was a decrease in the number and morphological changes in red blood cells, as well as an increase in the number of eosinophils. Hypochromic anemia and lymphocytopenia were found in the Se 2 MPC group. Conclusion: During an acute experiment, when selenium is given to rats at a dosage equal to the maximum permissible concentration and more, changes in the blood formula are observed, reflecting the pathological processes related to selenium poisoning: anemia, eosinophilia, lymphocytopenia.