Open AccessSTUDY OF THE ANTIOXIDANT ACTIVITY OF HESPERIDIN UNDER DEBILITATING PHYSICAL EXERTION IN RAT
Author(s) -
Anastasia Gerashchenko,
Natalia Shabanova
Publication year - 2022
Publication title -
archiv euromedica
Language(s) - English
Resource type - Journals
eISSN - 2199-885X
pISSN - 2193-3863
DOI - 10.35630/2199-885x/2022/12/2.11
Subject(s) - catalase , malondialdehyde , hesperidin , superoxide dismutase , antioxidant , lipid peroxidation , chemistry , pharmacology , medicine , endocrinology , biochemistry , pathology , alternative medicine
The antioxidant activity of hesperidin was studied against the background of debilitating physical activity. Materials and methods: Exhausting physical activity was reproduced in rats in the «Forced Swimming» test. A compound used in the study was administered at a dose of 100 mg/kg for 10 days of swimming. Results and discussions. In rats of the negative control group after 10 days of forced swimming, compared with intact animals, there was an increase in the activity of lipid peroxidation products in the blood: malondialdehyde and diene conjugates by 282.2% (p<0.05) and 41.3% (p<0.05). In turn, there was a decrease in the activity of antioxidant defense enzymes: superoxide dismutase by 44.8% (p<0.05), catalase by 53.8% (p<0.05), GP by 34.5% (p<0.05) relative to the parameters of the intact group of rats. The use of hesperidin made it possible to correct these disorders — there was a significant increase in the activity of SOD, catalase and GP by 77.8% (p<0.05), 172.7% (p<0.05) and 153.5% (p<0. 05), respectively, and a decrease in the level of TBA-active products and DC in muscle tissue by 57.5% and 43.1% (p<0.05) relative to the negative control group. Against the background of the introduction of the comparison drug Mexidol, a significant decrease in the level of endogenous pro- and an increase in antioxidants was observed. Conclusion: The results of the study allow us to recommend this compound as a corrector for biochemical shifts that may occur during exhausting loads.