Open AccessSTUDY OF POSSIBLE MECHANISMS OF ACTOPROTECTIVE ACTION OF СATEKHIN HYDRATE
Author(s) -
Anastasia Gerashchenko,
Natalia Shabanova,
Marina Efremova,
Andrey Voronkov
Publication year - 2021
Publication title -
archiv euromedica
Language(s) - English
Resource type - Journals
eISSN - 2199-885X
pISSN - 2193-3863
DOI - 10.35630/2199-885x/2021/11/2/8
Subject(s) - chloral hydrate , enos , nitric oxide , chemistry , receptor , nitric oxide synthase , medicine , catechin , endocrinology , pharmacology , antioxidant , biochemistry , biology , polyphenol
A study was carried out to study the possible mechanisms of actoprotective action of catechin hydrate administered at a dosage of 100 mg/kg. The animals were subjected to daily exhausting loads in the forced swimming test with a load of 10% of the animal's weight. After the end of the experiment, the rats were decapitated under chloral hydrate anesthesia (350 mg/kg) and the skeletal muscle was taken to obtain the supernatant. Using the ELISA method, the concentrations of nitric oxide isoforms (eNOS, iNOS, nNOS), PPAR (peroxisome proliferator-activated receptors) and JNK were estimated. It was found that against the background of the introduction of the test substance, an increase in eNOS activity was observed by 48.7% (p <0.05) relative to the group of negative control rats, as well as a decrease in iNOS and JNK — 1.9 times (p <0.05). In comparison with the group receiving Metaprot®, the concentration of endothelial synthase in the group receiving catechin hydrate was 1.3 times higher (p <0.05). The experiment performed suggests that the actoprotective effect of catechin hydrate is likely to be associated with inhibition of pathways mediated by JNK, activation of PPAR receptors, and the effect of these compounds on NO isoforms.