Open AccessCHARACTERISTIC OF HOSPITAL KLEBSIELLA PNEUMONIAE STRAINS CIRCULATING IN THE PEDIATRIC HOSPITAL
Author(s) -
Irina V. Belova,
A.G. Tochilina,
И. В. Соловьева,
О. В. Ковалишена,
И Ю Широкова,
Л. Ю. Послова,
В. А. Жирнов,
С. Б. Молодцова
Publication year - 2019
Publication title -
zdorovʹe naseleniâ i sreda obitaniâ
Language(s) - English
Resource type - Journals
eISSN - 2619-0788
pISSN - 2219-5238
DOI - 10.35627/2219-5238/2019-317-8-25-29
Subject(s) - microbiology and biotechnology , klebsiella pneumoniae , aerobactin , biology , antibiotics , fosfomycin , nitrofurantoin , antibiotic resistance , gene , genetics , enterobacteriaceae , escherichia coli
We researched 26 K. pneumoniae ssp. pneumoniae strains, isolated from patients and objects of the environment during the epidemic ill-being period in the pediatric hospital. Identification of strains and their clustering, study of antibiotic sensitivity, detection of antibiotic resistance determinants, whole genome sequencing were carried out.It is established that one strain characterized by identical RAPD patterns and sensitivity to antibiotics from the groups of cephalosporins, monobactams, aminoglycosides, carbapenems, fluoroquinolones, sulfonamides and glycylcyclides circulates in the department.The strain is resistant to ampicillin (MIC > 16) and nitrofurantoin (MIC > 64). Whole genome sequencing made it possible to characterize the strain as K. pneumoniae ssp. pneumoniae ST 3181K-54, the analysis of the whole genome sequence showed the presence of a fosomycin resistance gene (fosA) and virulence determinants namely a complex of siderophores (aerobactin and enterobactin), fimbria of the third type, iron utilization system kfu and cluster of genes responsible for allantoin utilization that allowed us to consider the strain as a microorganism with a high pathogenic potential capable to rapid prevalence and dissemination in tissues.