Open AccessDEVELOPMENT OF A METHOD FOR IDENTIFYING OF V. PARAHAEMOLYTICUS BY REAL-TIME PCR
Author(s) -
O.A. Rykovskaya,
М. В. Полеева,
О. С. Чемисова,
A. L. Trukhachev
Publication year - 2018
Publication title -
zdorovʹe naseleniâ i sreda obitaniâ
Language(s) - English
Resource type - Journals
eISSN - 2619-0788
pISSN - 2219-5238
DOI - 10.35627/2219-5238/2018-300-3-48-50
Subject(s) - vibrio parahaemolyticus , halophile , real time polymerase chain reaction , microbiology and biotechnology , biology , identification (biology) , selection (genetic algorithm) , computational biology , polymerase chain reaction , gene , bacteria , genetics , computer science , ecology , artificial intelligence
Bacteria of the species V. parahaemolyticus are halophilic microorganisms that may be causing acuteenteric disease in humans, occurring according to the type of foodborne illness. The success of thetreatment-and-prophylactic and sanitary-epidemiological measures aimed at the prevention of theproliferation of «halophiles» largely depends on timely and accurate identification of strains of Vibriosecreted from patients, seafood, as well as in monitoring of environmental objects. This paper presents amethod of identifying strains of the species V. parahaemolyticus by PCR in «real time» (Real-TimePCR), which enables the selection of chromosomal DNA from the test material, the PCR with thedesigned by us primers specific to the portion of a gene collagenase vppC and the probe, allowing todetect amplificatory the fragment and to incorporate the results in real-time.