Open AccessPREVALENCE OF ICE ELEMENTS OF DIFFERENT TYPES IN V. CHOLERAE
Author(s) -
С. О. Водопьянов,
А. С. Водопьянов,
Igor Paulovich Oleynikov,
С. В. Титова
Publication year - 2018
Publication title -
zdorovʹe naseleniâ i sreda obitaniâ
Language(s) - English
Resource type - Journals
eISSN - 2619-0788
pISSN - 2219-5238
DOI - 10.35627/2219-5238/2018-298-1-33-35
Subject(s) - vibrio cholerae , serotype , cholera , biology , microbiology and biotechnology , cholera toxin , genome , virology , genetics , bacteria , gene
With the help of a virtual PCR in silico analysis of the local database 395 genome-nucleotide sequencesof Vibrio cholerae in the presence of ICE element. The results showed widespread ICE member fromtoxigenic strains and nontoxigenic Vibrio cholerae O1 serotype. Thus all elements of ICE successfullytyped three basic types – «Indian», «Mozambique», or «the SXT». In this case up to 40 % nontoxigenicstrains O1 serovar and nonO1/nonO139 carried ICE element. When analyzed by PCR in vitrocollection of 222 strains of Vibrio cholerae O1 serotype shows the presence of ICE element and determineits type in toxigenic strains isolated in recent years in the Russian Federation. All nontoxigenic O1,O139 and nonO1/nonO139 strains isolated in Russia, deprived of ICE element that differs from strainscirculating in endemic cholera areas. This observation allows us to consider the presence of ICE elementas a new epidemic marker «of invasive strains of» nontoxigenic Vibrio cholerae.