Open AccessPartículas ambientales en bronquio y tejido pulmonar humano
Author(s) -
Laura Peña-García,
Roberto Maciel-Flores,
Celia Robles-Murguía,
José Rosas-Elguera
Publication year - 2019
Publication title -
revista de ciencias de la salud
Language(s) - English
Resource type - Journals
ISSN - 2410-3551
DOI - 10.35429/johs.2019.18.6.10.17
Subject(s) - scanning electron microscope , materials science , phosphate buffered saline , substrate (aquarium) , sputtering , glutaraldehyde , nanotechnology , composite material , chemistry , chromatography , thin film , oceanography , geology
Objectives. Identify environmental particles in bronchus and human lung tissue through the SEM technique Methodology. The samples were fixed by immersion in 2% glutaraldehyde for 2 hours, then a wash with 1x pH 7 phosphate buffer and light agitation (x3) (1x, 2x and x3) was performed. Finally, the samples were critically dried out with a Samdri 795 team from Tousimis. This was the process to which the samples were taken for EDS analysis with a Jeol JSM 6610LV equipment, operating at 10kV, with Oxford Xmax EDS detector and Oxford AZtec software. The observation in the scanning electron microscope was performed with secondary electron detector. For the assembly, a sample holder was used for scanning electron microscopy, on a double-sided carbon tape substrate. The samples were given a coating with conductive material (99.9% gold) by sputtering with the Denton Vacuum V equipment. Contribution. In lung, 21 elements were identified, in addition to observing the shape and size of the particles. These images were compared with some other environmental particles presented by diverse authors. The uses of these elements were consulted and it was identified that some of them are used in the automotive, electrical and medical industries, and they also have a great influence on food.