Open AccessSTUDY ON FACTORS AFFECTING THE FLOWERING OF FORGET-ME-NOT (Browallia americana L.) IN VITRO CULTURE
Author(s) -
Linh Thi Thuy Le,
Thuong Tieu Linh Tran,
Hung Duc Le,
Dien Huynh Han,
Thy Thi Bich Le,
Thuc Van Le
Publication year - 2020
Publication title -
khoa học
Language(s) - English
Resource type - Journals
ISSN - 1859-4816
DOI - 10.35382/18594816.1.40.2020.613
Subject(s) - biology , explant culture , sugar , plant growth , shoot , horticulture , tissue culture , botany , in vitro , photoperiodism , food science , biochemistry
Vegetative to reproductive transition depends on different factors. This study was conducted to examine factors affecting the growth and flowering of Browallia americana L. in vitro such as the age of the sample, mineral content, plant growth regulators, concentration and type of sugar, ventilation culture. The results showed that 40-day-old shoots were a suitable source of in vitro flowering (90.85% after 45 days of culture). Mineralcontent had a great influence on the ability to differentiate flower buds of plants, the highest in Murashige and Skoog medium (87.22% after 45 days of culture). Meanwhile, most of the plant growth regulators which were surveyed in this study inhibited the flowering. In particular, culture medium with glucose showed higher flowering efficiency than saccharose, the highest flowering rate was at 50 mg/L glucoses (90.52%, with 3.50 flower buds/explant after 30 days of culture). In addition, ventilation culture, which used plastic wrapedwith milipore filter had the highest rate of flower bud formation (average 3.58 flower buds/plant). This result is an important foundation for studying the flowering of in vitro plant culture, which will be one of the great paradigm plants for teaching and basic research.