ACREDITED MOLECULAR METHODS FOR DETECTION OF INFECTIONS CAUSED BY HIV, HEPATITIS B VIRUS AND HEPATITIS C VIRUS

Hepatitis B and C, as well as HIV infections are common pathology in present time. According current assessments 350 million are carriers of Hepatitis B virus, 170 million of Hepatitis C virus and over 60 million infected by HIV. Timely detection and prompt diagnosis of these viral infections are of great importance.According assessments, in our country about 1 of 50 persons are infected by Hepatitis B or Hepatitis C. From the beginning of the epidemic in 1987, until 2016 total number of registered of HIV/AIDS is 311 (179 with AIDS and 131 HIV positive).In JZU - University Clinic for Infectious Diseases and Febrile Conditions Skopje, diagnosis of these viral infections are made by accredited molecular diagnostics methods, PCR, at Department for molecular diagnostics using HCV-RNA, HBV-DNA and HIV-RNA. Aims of accreditation of particular procedures in laboratories is protection and advancing of human health, and preventing of spreading of viral infections.With great success, Department for molecular diagnostics as part of the University Clinic For Infectious Diseases and Febrile Conditions Skopje use the System for quality management according standard MKS EN ISO 15189:2013 for medical laboratories. Accredited molecular methods in use for detection of Hepatitis C virus, Hepatitis B virus and HIV are Polymerase Chain Reaction (PCR) and Genotypisation of HCV with method of Reverse hybridization.