Open AccessBiomechanical evaluation of central and peripheral Descemet’s membrane endothelial graft
Author(s) -
Vito Romano,
Zhuola Zhuola,
Zhuo Chang,
Bernhard Steger,
Hannah J. Levis,
Stephen B. Kaye,
Riaz Akhtar
Publication year - 2018
Publication title -
journal for modeling in ophthalmology
Language(s) - English
Resource type - Journals
eISSN - 2468-3930
pISSN - 2468-3922
DOI - 10.35119/maio.v2i2.71
Subject(s) - cornea , peripheral , ophthalmology , descemet's membrane , descemet membrane , corneal transplantation , transplantation , medicine , materials science , surgery
Corneal endothelial transplant is the gold-standard treatment in cases of corneal endothelial cellular dysfunction. Preparation, delivery, and unfolding of the graft are technically demanding. We assessed the biomechanical behavior of Descemet’s membrane to better understand Descemet’s membrane endothelial keratoplasty(DMEK) graft behavior, and to select the right diameter and donor age graft . The biomechanical behavior was tested using atomic force microscopy (AFM) on five corneas unsuitable for transplantation. The peripheral cornea was found to be stiffer than the central cornea (3171.89 MPa and 2837.20 MPa, respectively). The elastic modulus of both the central and peripheral cornea exhibited a trend to decrease with age. In addition, the central cornea becomes stiff er than the peripheral cornea in older patients, while the peripheral cornea was stiff er in younger patients. AFM is a suitable technique for evaluating biomechanical behavior of DMEK graft s. One interpretation of this varied behavior is that the type and quantity of collagen changes with age and location.