Open AccessInvestigation of metabolites produced by Magnaporthe oryzae during appressorium development using 1H NMR metabolomics approach
Author(s) -
Azian Md Zain,
Nur Ain Izzati Mohd Zainudin,
Intan Safinar Ismail,
Amalina Ahmad Azam,
Wan Zuhainis Saad,
Mohd Termizi Yusof
Publication year - 2021
Publication title -
asia-pacific journal of molecular biology and biotechnology/asia pacific journal of molecular biology and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2521-9839
pISSN - 0128-7451
DOI - 10.35118/apjmbb.2021.029.3.09
Subject(s) - valine , metabolite , biochemistry , alanine , biology , appressorium , chemistry , metabolome , amino acid , xylose , fermentation , gene
This study was aimed to determine metabolites produced by Magnaporthe oryzae and identify metabolic changes during appressorium development. Appressorium development were induced in vitro and subjected to 1H NMR spectroscopy for metabolites production and multivariate data analysis. PCA, PLS-DA and OPLS-DA were used to profile metabolite production throughout appressorium development. There were 43 metabolites identified putatively and PCA showed differences of metabolites production between mycelium and appressorium development. Metabolites that were significantly produced (p < 0.05) during appressorium development including isocitrate, isobutyrate, lysine, glutamate, succinate, tyrosine, choline, glycerol, xylose, mannose, sucrose, tryptophan, butyrate, leucine, isoleucine, valine, ethanol, methylmalonate, threonine, lactate, alanine, arginine, 4-aminobutyrate, homoserine, glucose, mannitol and glucitol. Glycerolipid, carbohydrates and amino acids metabolisms showed to be highly involved during appressoria development. This study revealed metabolites produced by M. oryzae during appressoria development in vitro as first metabolomics data using 1H NMR approach.