Open AccessAnalysis of DNA methylation level and mRNA expression of Transient Receptor Ankyrin Member 1 (TRPA1) in endometriosis-associated pain
Author(s) -
Ocktariyana Ocktariyana,
Nurul Hikmawati,
Andon Hestiantoro,
Muharam Natadisastra,
M. Luky Satria Marwali,
Agus Surur,
Tiara Aninditha,
Gita Pratama,
Anisah Zahrah,
Nabilla Farah Naura,
Asmarinah Asmarinah
Publication year - 2021
Publication title -
asia-pacific journal of molecular biology and biotechnology/asia pacific journal of molecular biology and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 2521-9839
pISSN - 0128-7451
DOI - 10.35118/apjmbb.2021.029.3.01
Subject(s) - endometriosis , dna methylation , transient receptor potential channel , ankyrin , methylation , real time polymerase chain reaction , medicine , messenger rna , gene expression , receptor , gene , microbiology and biotechnology , endocrinology , biology , genetics
Transient Receptor Ankyrin Member 1 (TRPA1) is an ion channel family protein that regulates pain sensation through sensory neurons' activity. This study's purpose to analyzes the DNA methylation and mRNA expression level of the TRPA1 gene in endometriosis and its correlation with pain level. Twenty samples of peritoneal endometriosis and endometrial samples were obtained from women with endometriosis, which was subsequently compared to 20 endometrial samples of women without endometriosis. The DNA methylation level of TRPA1 was analyzed using Methylation-specific PCR (MS-PCR) and ImageJ software, while the mRNA expression of TRPA1 was analyzed using qRT-PCR. Furthermore, the pain level was measured using the numeric rating scale (NRS) by interviewing all the women. This study showed that there was a significant difference in the mRNA expression of TRPA1 in peritoneal endometriosis. The TRPA1 was unmethylated in both peritoneal and endometrial samples in endometriosis. However, DNA Methylation level of TRPA1 in peritoneal and endometrial of endometriosis compared to normal endometrial were no significant difference. Additionally, there was no correlation between DNA methylation level and mRNA expression level of TRPA1 in all samples, along with the endometriosis-associated pain.