Open AccessExperimental justification of the effectiveness of cord blood stem cell transplantation in chronic myocardial injury
Author(s) -
A. V. Gabrielyan,
T. Domansky
Publication year - 2019
Publication title -
sučasnì medičnì tehnologìï
Language(s) - English
Resource type - Journals
ISSN - 2072-9367
DOI - 10.34287/mmt.2(41).2019.4
Subject(s) - transplantation , medicine , xenotransplantation , stem cell , cord blood , regeneration (biology) , andrology , surgery , biology , genetics , microbiology and biotechnology
Purpose of the study. Determination oftransplantation efficiency of cord blood stemcells (CB SC) for damage to the myocardium andassessment of the repair of its disturbed functionsin the experiment.Materials and methods. The experiment wasconducted on mice of the FVB line at the age of 5 months,with a body weight of 25–30 g. Animals were dividedinto experimental groups of 5 animals in each. Theanimals of the study group were derived from theexperiment before the SC suspension injection, after2 days, 4 and 8 weeks after cell transplantation.Results. Analysis of morphofunctional changesafter the introduction of cord blood cells in 4 and8 weeks compared to the original state (3 weeksafter modeling of the isoproterenol inducedmyocardial injury model) has established a cleartendency to improve the investigated parameters.The confirmed phenomenon of self-referral of cordblood nto the lesion zone proves the expediencyof transplantation by intravenous injection of asuspension of CB SC. It has been established thattransplantation of CB SC causes a temporarydeterioration of morphofunctional features, whichmay be a manifestation of the body's response toxenotransplantation. The CB SC transplantationhas a positive effect on the isoproterenol-inducedmyocardial injury model.Conclusions: 1. Accumulation of CBSC in thezone of injury after transplantation, proved byexperimental research. 2. CB SC transplantationaccelerates the processes of myocardial regeneration on the model of injury in laboratory animals. 3.Intravenous injection of the CB SC suspension to themodels of myocardial injury proved to be effective.4. The experimental results obtained can serve asthe basis for further clinical studies in patients withheart failure.Keywords: stem cells, transplantation, model injury, umbilical cord blood.