Open AccessActive Fractions of Methanol Crude Obtained from Acacia Seyal Gum and their Antiproliferative Effects against Human Breast Cancer Cell Lines
Author(s) -
Ahmed Am Elnour,
Mohamed Elwathig Saeed Mirghani,
N. A. Kabbashi,
Djabir Daddiouaissa,
Khalid Hamid Musa,
Zahangir Alam,
Nour Hamid Abdurahman
Publication year - 2020
Publication title -
global journal of science frontier research
Language(s) - English
Resource type - Journals
eISSN - 2249-4626
pISSN - 0975-5896
DOI - 10.34257/gjsfrcvol20is3pg51
Subject(s) - chemistry , acacia , human breast , methanol , chromatography , exudate , traditional medicine , cancer cell lines , acetone , botany , food science , cancer , biology , biochemistry , cancer cell , medicine , organic chemistry , genetics
Background: This study is on Acacia seyal gum, which is an exudate from Talha (Acacia seyal) tree. It provides a rich source of prebiotic that is used traditionally in folk medicine. Aims: The anti-proliferative effect (APE) of Acacia seyal gum (ASG) and Prebio-T-commercial (PTC) samples on human breast cancer (MCF-7) cell lines, and their antioxidant activities (AA) were investigated. Methods: The methanol crude extracts of both Acacia seyal gum and Prebio-T- commercial were fractioned into acetone and methanol, respectively. The anti-proliferative effect on human breast cancer cell lines for each fraction was examined using sulphorhodamine assay (SRB assay). Methanol crude extracts and their active compositions were analysed carefully using Gas chromatography-mass spectrometry technique.