Open AccessRegulation of Specific Cell Clusters in TCR-T Cells Responding to Differential Expression of Tumor PD-L1
Author(s) -
Renpeng Ding,
Shang Liu,
Huanyi Chen,
Bin Kang,
Radoje Drmanac,
Yixin Gu,
Xuan Dong,
Qianqian Gao
Publication year - 2020
Publication title -
global journal of medical research
Language(s) - English
Resource type - Journals
eISSN - 2249-4618
pISSN - 0975-5888
DOI - 10.34257/gjmrfvol20is8pg11
Subject(s) - t cell receptor , cd8 , cytotoxic t cell , cd38 , cd28 , t cell , cytotoxicity , tumor microenvironment , microbiology and biotechnology , downregulation and upregulation , biology , chemistry , cancer research , antigen , immunology , immune system , in vitro , biochemistry , stem cell , gene , cd34
PD-L1 signaling is essentialin regulating T cell function and keeping the balance of tumor microenvironment, but its role in modifying TCR-T cell cytotoxicity remains unknown. MART-1-specific TCR-T cells (TCR-TMART-1) were stimulated by MEL-526 tumor cells expressing different proportions of PD-L1 and used to perform cytotoxicity assays and single-cell RNA sequencing. Percentage changes of different specific cell clusters were analyzed. The percentage of cluster HLA-DR+CD38+CD8+was upregulated after antigen stimulation, and tumor PD-L1 modified TCR-T cell function through down regulating the percentages of HLA-DR+CD28+CD8+and HLA-DR+CD38+CD8+subsets which were higher in TCR-TMART-1than in Tnull.