Open AccessSynthesis of Ag-NPs from extracts of Persea americana and its antimicrobial effects in human pathogens
Author(s) -
Selvam K.A,
M Suriyakumar,
J Devanathan,
K. Kolanjinathan,
A. Ram Kumar,
Selvakumar Selvaraj,
L Ramadass
Publication year - 2020
Publication title -
nanonext
Language(s) - English
Resource type - Journals
ISSN - 2582-8622
DOI - 10.34256/nnxt2012
Subject(s) - streptococcus mutans , antimicrobial , aspergillus niger , serratia marcescens , persea , silver nanoparticle , candida albicans , agar diffusion test , chemistry , phytochemical , antibacterial activity , microbiology and biotechnology , biology , traditional medicine , nuclear chemistry , escherichia coli , food science , bacteria , botany , materials science , nanoparticle , biochemistry , medicine , genetics , gene , nanotechnology
Medicines of plant origin have been used to combat human diseases since time immemorial even before the history of their documentation. They have been used by human beings since time immemorial for curing health. The phytochemical screening of leaf extracts of Persea americanashows the presence of alkaloids, flavanoids, carbohydrates, saponins, protein, tannins and glycoside. Also the synthesis of silver nanoparticles was done using ethanolic Persea americana leaf extract. The synthesized silver nanoparticles were characterized by using Ultraviolet- visible (UV-Vis) spectroscopy, X-ray diffraction analysis (XRD), scanning electron microscopy (SEM) and Fourier transform infrared (FT-IR) analysis. The antimicrobial potential of synthesized nanoparticles was determined on the basis of mean diameter of zone of inhibition around the disc in millimeters. The antibacterial activity was evaluated qualitatively through agar disc diffusion towards Lactobacillus sp, Staphylococcus aureus, Streptococcus mutans, Klebsiella pneumoniae, Serratia marcescens, and Escherichia coli. The highest zone of inhibition value (15.0 mm) in Streptococcus mutans 15 ± 0.6. The antifungal activity against Aspergillus niger, Aspergillus flaves ,Candida albicans and Fusarium sp. From the results, excellent and eco-friendly green source for production of potential bio antimicrobial silver nanoparticles.