Open AccessBIOSTIMULATOR MADE OF WASTE OF KERATIN-CONTAINING RAW MATERIALS
Author(s) -
Лариса Брындина
Publication year - 2020
Publication title -
lesotehničeskij žurnal
Language(s) - English
Resource type - Journals
ISSN - 2222-7962
DOI - 10.34220/issn.2222-7962/2020.1/1
Subject(s) - raw material , hydrolysis , amino acid , keratin , chemistry , food science , yield (engineering) , enzymatic hydrolysis , fermentation , pulp and paper industry , biochemistry , waste management , organic chemistry , biology , materials science , paleontology , metallurgy , engineering
Ecosystem degradation forces us to introduce highly productive, environmentally friendly biotechnologies in agricultural production. In this regard, interest in organic farming is growing. Demand for organic products is ahead of supply. This, in its turn, causes interest in substances that, on the one hand, are safe for the environment, and on the other hand, increase yield and quality of crops. These substances include biostimulants. They stimulate the natural processes of assimilation of nutrients, increase stress resistance of plants. In this regard, keratin-containing raw materials deserve special attention, which, in terms of chemical composition, is a protein concentrate (90-95%) . But its use is limited due to the low functionality of the keratin protein of this raw material. Enzymatic keratin hydrolysis will provide a biostimulant for plants with a high content of amino acids. The aim of the study was to develop an enzymatic method for keratin hydrolysis with a high content of amino acids in the final product. Fermentation was carried out by Str. chromogeness.g. 0832 proteinase. The content of soluble protein, peptides and amino acids was carried out photocolorimetrically. Temperature (4000C) and pH (8.0) conditions of Str. chromogeness.g. 0832 enzyme complex have been determined, which allowed further hydrolysis of keratin-containing raw materials with a maximum yield of final products - amino acids (99.57 g/100g). Treatment of corn seeds with the obtained amino acid solution enabled to increase growth-promoting activity by 48%.