Open AccessProtein linking fatty acids and its genetic regulation in children having food allergy
Author(s) -
Н. Г. Приходченко,
Т. А. Шуматова,
С. В. Воронин,
D.V. Kovalenko
Publication year - 2022
Publication title -
tihookeanskij medicinskij žurnal
Language(s) - English
Resource type - Journals
ISSN - 1609-1175
DOI - 10.34215/1609-1175-2021-4-46-49
Subject(s) - urine , genotype , feces , exacerbation , allele , food allergy , pathological , allergy , biology , polymorphism (computer science) , gene , immunology , medicine , genetics , microbiology and biotechnology
Objective: Study the effect of the Ala54Thr FABP gene polymorphism on the produce of the intestinal FABP fraction in blood serum. urine and coprofiltrate in children having food allergies. Methods: The content of the FABP intestinal fraction in urine, feces, and blood serum was determined using ELISA method. The study of FABP genes polymorphism (G163A, Ala54Thr) was carried out using PCR method. Results: Statistically significant increase of the FABP level in blood serum, urine and feces in children with FA was detected in various biological fluids. The distribution of FABP2 alleles and genotypes obeyed the Hardy-Weinberg law (χ 2 = 0; p = 1,000) and did not significantly differ from the distribution of genotypes in children having FA andin the control sample (p = 0.638). Conclusions: The study did not reveal an association of the pathological genotype FABP G163A, (Ala54Thr) with the hyperproduction of the FABP intestinal fraction in children having FA, confirming the diagnostic significance of this marker increase during exacerbation of the disease.