Open AccessAntitumor activity of pectins of various molecular weights
Author(s) -
Ekaterina Leonteva,
V. V. Vikhareva,
A. A. Kalitnik,
Maksim Khotimchenko
Publication year - 2020
Publication title -
tihookeanskij medicinskij žurnal
Language(s) - English
Resource type - Journals
ISSN - 1609-1175
DOI - 10.34215/1609-1175-2020-4-68-72
Subject(s) - cytotoxic t cell , propidium iodide , adenocarcinoma , chemistry , cytotoxicity , hela , cell culture , cancer research , biochemistry , mtt assay , cell , cancer cell , biological activity , in vitro , cancer , apoptosis , microbiology and biotechnology , biology , programmed cell death , genetics
Objective: Comparative analysis of the antitumor characteristics of different pectins and the dependency of their biological effects on the structural features and the molecular weight. Methods : Cell lines of the murine adenocarcinoma of the mammary gland C127, carcinoma of the cervix HeLa, adenocarcinoma of the colon SW620, adenocarcinoma of the mammary gland HTB-30 and the normal cells of the retina RPE were used to study the antitumor activity of the polysaccharides. The assessment of the antitumor and cytotoxic actions of the pectins was made by testing metabolic activity through MTT-assay and by making intravital fluorescent coloring of the live and dead cells using DNA-connecting color Hoechst and propidium iodide. Results : The samples of the citrus pectins in the selected concentration range had a sufficient antitumor effect on the adenocarcinoma C127 cell line by decreasing metabolic activity and the cell proliferation, and also by providing cilinically important cytotoxic effect. Conclusions : The data got in this study demonstrates that the reaction of the cancer cells on pectins depends on the type of the connection used and also on the sensitivity of the cells to pectins.