Open AccessAvaliação da proliferação celular de clostridium perfringens em intestino delgado de ruminantes / Evaluation of cellular proliferation of clostridium perfringens in small intestine of ruminants
Author(s) -
Simone Miyashiro,
Alessandra Figueiredo de Castro Nassar,
Renata Haddad Esper,
Nílson Roberti Benites
Publication year - 2021
Publication title -
brazilian journal of animal and environmental research
Language(s) - English
Resource type - Journals
ISSN - 2595-573X
DOI - 10.34188/bjaerv4n3-092
Subject(s) - clostridium perfringens , biology , microbiology and biotechnology , typing , isolation (microbiology) , clostridium , veterinary medicine , bacteria , genetics , medicine
This study evaluated the isolation, molecular typing and celular proliferation of C. perfringens in small intestine of cattle and sheep distributed in case and control groups. In the case group, out of the 61 bovine samples analyzed, C. perfringens was isolated in 29 (47.54%) and in 4 (33.34%) from 12 sheep samples. In the control group, we did not isolate the microorganism in the bovine samples (73 samples), however it was possible in 5 (20.83%) out of the 24 sheep samples. All isolates (100%) were classified as type A, and cell quantification showed that in control group, all cattle samples resulted in 10 CFU/g of intestinal content while in the study group presented a median value of 104 CFU/g, varying from 10 CFU/g through 108 CFU/g. In ovine group there was a clear divison of values between the two groups. Molecular typing corroborates with other studies regardin the epidemiological importance of type A in enterotoxaemia in ruminants, thus cellular quantification data allow us to conclude that healthy animals present a basal level of C. perfringens lower than 10 CFU/g that does not allow its isolation.