Open AccessPrevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran
Author(s) -
Mahtab Hamidpour,
Saman Mahdavi
Publication year - 2020
Publication title -
international journal of enteric pathognes/international journal of enteric pathogens
Language(s) - English
Resource type - Journals
eISSN - 2345-3362
pISSN - 2322-5866
DOI - 10.34172/ijep.2020.17
Subject(s) - bacillus cereus , cereus , raw milk , biology , food poisoning , polymerase chain reaction , enterotoxin , food science , microbiology and biotechnology , contamination , food contaminant , bacteria , diarrhea , gene , escherichia coli , medicine , genetics , ecology
Background: Bacillus cereus is a gram-positive and spore-forming bacterium which is widespread in nature. It also has been known as a major foodborne pathogen that often plays a role in the contamination of ready-to-eat and dairy products. It causes two different types of food poisoning in human: the diarrheal type and the emetic type. Objective: The current study was planned to determine the prevalence of ces and cytk genes of Bacillus cereus isolated from raw milk in Tabriz, Iran. Materials and Methods: In this study, 40 B. cereus strains isolated from cow raw milk, that had already been identified phenotypically, were assessed for molecular confirmation by polymerase chain reaction (PCR) method. Then, they were evaluated for presence of ces and cytK genes by specific primers. Results: Of 40 B. cereus strains, 39 strains were confirmed molecularly. The frequency of cytK and ces genes was reported 38 (97.43%) and 0 (0%), respectively. Conclusion: The results of present study showed that B. cereus strains isolated from raw milk had high potential in causing diarrhea poisoning. Therefore, using procedures to reduce the bacterial contamination during the processing of dairy product is essential.