Open AccessGenetic diversity study of Hungarian honey bee (Apis mellifera L.) colonies – Previous announcement
Author(s) -
Erika Zakar,
János Oláh,
András Jávor,
Szilvia Kusza
Publication year - 2012
Publication title -
acta agraria debreceniensis
Language(s) - English
Resource type - Journals
ISSN - 2416-1640
DOI - 10.34101/actaagrar/48/2454
Subject(s) - honey bee , microsatellite , breed , mitochondrial dna , biology , genetic diversity , honey bees , zoology , dna sequencing , veterinary medicine , evolutionary biology , genetics , ecology , dna , allele , demography , population , medicine , gene , sociology
In Hungary, the Apismelliferacarnica is the native breed which is the only recognised and breedable honey bee in the country. It is assumed that there are a number of non-native and hybrid honey bee breeds in Hungary. The microsatellite and mitochondrial DNA surveys applied here will be utilised to easily and accurately identify the various sprads, and open up new ways in the research of honey bees. The isolation of the genomial DNS from 5 to 7 day old larvae samples was successfully carried out. In the future the plan is to carry out the measure with microsatellite markers. As an initial step the optimal annealing temperature was identified. In the mitochondrial DNA survey the COI-COII mytochonrial regional primer due to its low anneling temperature cannot be used with any normal sequencing methods. By using these method the aim of this research is the measurement of genetic variance.