Open AccessAnalysis of longevity in Holstein Friesian cattle using proteomic approaches
Author(s) -
Gabriella Gulyás,
Béla Béri,
András Jávor,
László Márk,
Éva Csősz,
Krisztina Pohóczky,
Beáta Soltész,
Dániel Kuti,
Levente Czeglédi
Publication year - 2012
Publication title -
acta agraria debreceniensis
Language(s) - English
Resource type - Journals
ISSN - 2416-1640
DOI - 10.34101/actaagrar/48/2447
Subject(s) - longevity , biology , polyacrylamide gel electrophoresis , milk protein , zoology , dairy cattle , reproduction , proteomics , andrology , genetics , biochemistry , food science , enzyme , medicine , gene
The aim of the present study was to determine marker proteins those are associated with functional longevity of dairy cattle. Holstein-Friesian cows were grouped based on their performance as follows: group 1) individuals with good longevity traits; group 2) short production life because of poor reproduction traits; group 3) short production life with low milk yield. Twelve individuals were sampled in each group, blood and milk samples were collected from cows. Blood samples were analysed with two dimensional polyacrylamide gel electrophoresis (2D PAGE), MALDI TOF/TOF and nanoLCMS/MS. The milk samples were analysed with MALDI TOF/TOF and nanoLC-MS/MS. Using the optimized gel based proteomic approach,we have succesfully separated 143 proteins in the group1, 139 proteins in the group2 and 136 proteins in the group3, but we could not find significant differences between groups in the expression pattern. Using MALDI TOF/TOF and nanoLC-IonTrap MS, we have found eleven protein sequences those were expressed only in the samples of good longevity group.