Open AccessInvitro antimicrobial efficiency of Lawsonia inermis L (Henna) extracts against Multidrug Resistant Microorganisms
Author(s) -
Summra Javaid,
Amer Jamil,
Hina Awais,
Hafiz Muhammad Rehman,
Salman Shahid,
Saira Khan
Publication year - 2021
Publication title -
abasyn journal of life sciences
Language(s) - English
Resource type - Journals
eISSN - 2663-1040
pISSN - 2616-9754
DOI - 10.34091/ajls.4.2.7
Subject(s) - lawsonia inermis , antimicrobial , multiple drug resistance , biology , fusarium solani , traditional medicine , microbiology and biotechnology , agar diffusion test , drug resistance , bacteria , antibacterial activity , medicine , genetics
Antibiotics are becoming less effective as drug resistance spreads throughout the world, making it ever more difficult to treat the disease. The effect of biotic stress on antimicrobial activity of Lawsonia inermis L. (Henna) was studied as an alternate to antimicrobial agents against multidrug resistant microorganisms. The study was carried out in Molecular Biochemistry Lab, Department of Biochemistry, University of Agriculture Faisalabad. The plant seedlings were given biotic stress with Fusarium solani. The induced extracts were harvested at different time intervals and the antimicrobial activity was investigated by disc diffusion method. The induced plant extracts revealed highest susceptibility against S. aureus (30+-0.02) and Pasteurella multocida (30+-0.01) at 12 hpi. The least activity was shown against all the strains at 0 hpi. The highest antifungal activity was found against Ganoderma lucidum (55+-0.05) at 24 hpi. The results demonstrated a significant difference in the antimicrobial activities of all the strains with and without fungal stress (P<0.05). The fungal induced extracts of Lawsonia inermis L (Henna) with enhanced antimicrobial activity may have the potential of being alternative and cost- effective agents against antimicrobial resistance.Keywords: Antimicrobial; In-vitro; Lawsonia inermis; Drug resistance; Antibiotics.