Detection of Cortisol Level via Enzyme Immunoassay Influenced by Exercise

Cortisol is among the human body’s most necessary hormones. On anygiven day, cortisol’s primary function is to control how our body createsand utilizes energy. This study examined the influence of exercise on thecortisol levels.Twenty female subjects had their cortisol levelsdetermined before their morning exercise (Pre) and after their morningexercise (Post) using microplate enzyme immunoassay method. Theresult of study showed that there was significant difference (P<0.05) inthe cortisol levels before exercise 13.49 ± 0.90µg/dl Compared with afterexercise 15.10±1.08µg/dl. It was also observed that there was nosignificant difference in the cortisol level (P>0.05) obtained from thefemale subjects before and after exercise between the ages of 21-23, 24-26 and 27-29 years. Collectively, these results showed that exerciseprovoked increased in cortisol level.Keyword: Cortisol, Immunoassay, Exercise, Hormone