Isolation, Screening and Methionine Production by Bacillus species isolated from Poultry Waste

The current study was focused on isolating and identifying methionineproducing bacteria from poultry waste. The bacteria were isolated frompoultry waste and the ones which specifically produced methionine onsolid agar medium, using a methionine requiring auxotroph (Escherichiacoli) were kept for further analysis. Methionine assay was also carriedout after fermentation to quantify methionine produced by each isolate.A total of 10 bacteria were isolated of which 2 were confirmed as activemethionine producers after halo growth was observed on the solid agarmedium. These 2 isolates which produced higher amount of methioninewere ultimately selected for further investigation. The methionineproducing isolates which were designated PW1 and PW3 were identifiedas Bacillus sp. respectively after biochemical tests were carried out. Thetest carried out include; gram staining, motility test, catalase test, citratetest, hemolysis test, indole test, penicillin sensitivity, oxidase test andcrystal colony formation. Where PW3 (Bacillus sp.) produced greateramount of methionine (0.54 mg/l), while PW1 (Bacillus sp.) produced0.20 mg/l after the completion of the methionine assay.Keywords: Keywords: Methionine production, Bacillus species, poultrywaste.