Open AccessInvestigation of bacteria liable for Urinary Tract Infections and their antibiogram analysis
Author(s) -
Wajeeha Iqbal,
Mahrukh Khattak,
Muhammad İshaq,
Abdul Rehman,
Ghadir Ali
Publication year - 2018
Publication title -
abasyn journal of life sciences
Language(s) - English
Resource type - Journals
eISSN - 2663-1040
pISSN - 2616-9754
DOI - 10.34091/ajls.1.1.4
Subject(s) - levofloxacin , nitrofurantoin , imipenem , microbiology and biotechnology , medicine , ciprofloxacin , piperacillin , meropenem , amikacin , antibiotics , cefoperazone , fosfomycin , ampicillin , pseudomonas aeruginosa , biology , antibiotic resistance , bacteria , genetics
Urinary tract infection mainly occurs by microorganism when theyovercome the human immune system. These microbes have caused thehigh rate of morbidity and motility effect all over the world to every ageand gender. Lower urinary tract infection is more common amongfemales than males. A prospective observational study was conductedin Pathology Department at Lady Reading Hospital Medical TeachingInstitution, Peshawar, Pakistan in a 4th quarter (October, November,December) of the year 2017. Mid-stream urine samples were collectedfrom 1357 patients from all hospital-admitted and outdoor patients.They were cultured on CLED agar, after isolation, uropathogens wasidentified through biochemical tests like oxidase, catalase and TSI tests.Only 205 samples were reported positive, with the prevalence of15.10%. Females (51.70%) were more prone to UTI then males (48.29%).E.coli was most frequently isolated uropathogen with frequency of90.24%. Other isolates were Pseudomonas aeruginosa (4.8%),Enterococcus faecium (2.92%), Enterobacter species (1.46%) and Proteusmirabilis (0.49%). Thirteen antibiotics were used to test the susceptibilityof isolated microbes through Kirby Bauer Disc Diffusion method.Carbapenem drug like Imipenem (89.7%) was most effective againstgram-negative microbes belonging to Enterobacteriaceae family. Otherantibiotics like, Piperacillin + Tazobactam (84.86%), Cefoperazone/Sulbactam (84.3%), Amikacin (84.3%), Fosfomycin (83.7%) andNitrofurantoin (77.2%) also showed sensitivity. Fluoroquinolones Drugslike Ciprofloxacin (82.1%) and Levofloxacin (81.6%) showed highresistivity followed by Co-trimoxazole (80.54%) and Cephalosporinedrugs. For Gram positive Enterococcus spp, Glycopeptide antibiotics andprotein synthesis inhibitor drugs had shown 100% sensitivity, butcarbapenem and fluoroquinolones were highly resistant.Keywords: Enterobacteriaceae Family, Urinary Tract Infection (UTI),Fluoroquinolones Drug