Open AccessIsolation and Identification of Pathogenic Bacteria from Urinary Tract Infections in Infants from Different Hospitals of District Peshawar
Publication year - 2018
Publication title -
abasyn journal of life sciences
Language(s) - English
Resource type - Journals
eISSN - 2663-1040
pISSN - 2616-9754
DOI - 10.34091/ajls.1.1.3
Subject(s) - staphylococcus saprophyticus , microbiology and biotechnology , isolation (microbiology) , pathogenic bacteria , bacteria , gram staining , macconkey agar , nutrient agar , agar , coagulase , biology , urinary system , chocolate agar , medicine , staphylococcus aureus , staphylococcus , antibiotics , genetics
In the present study isolation and identification of pathogenic bacteria,causing urinary tract infection in infants was performed. The researchwork was carried out at the Microbiology Laboratory, Abasyn University,Peshawar. Samples were collected from different hospitals of DistrictPeshawar. The collected samples were inoculated on different media foridentification and isolation such as Nutrient agar, MacConkey agar,Mannitol salt agar and CLED media. For further identification Gramstaining and Biochemical tests were performed such as Catalase,Oxidase, Coagulase, Indole and TSI. In total of 90 samples, E. coli wasmost prominent, which was found to be 52 (57.77%) followed by S.saprophyticus 22 (24.44%), K. pneumonia 11 (12.22%).While leastprominent was S. aureus 5 (5.55%). Among the total isolates thepercentage of Gram-negative bacteria in UTI patients was higher thanGram-positive bacteria. Whereas the percentage of E. coli was (53%)and K. pneumonia was (14%) while the percentage of S. saprophyticuswas (28%) and S. aureus (5%). Among the total 90 isolates, the highestprevalence was observed in female infants about (58.88%) while in maleinfants, the percentage was (41.11%).Key words: Urinary Tract Infection, Infants, Pathogenic Bacteria.