Open AccessUK–Russia Researcher Links Workshop: extracellular vesicles – mechanisms of biogenesis and roles in disease pathogenesis, M.V. Lomonosov Moscow State University, Moscow, Russia, 1–5 March 2015
Author(s) -
Kapustin Alexander N.,
Kalinitalia,
Lopatina Tatiana,
Davidson Sean M.,
Iraci Nunzio,
Tamkovich Svetlana,
Smyth Lesley,
TerOvanesyan Dmitry,
Evtushenko Evgeniy G.,
Savelieva Olga,
Bertazzo Sergio,
Aushev Vassiliy,
Dragovic Rebecca,
Gracia Tannia,
Heck Margarete,
Parfyonova Yelena V.,
Shanahan Catherine M.,
Tkachuk Vsevolod
Publication year - 2015
Publication title -
journal of extracellular vesicles
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.94
H-Index - 68
ISSN - 2001-3078
DOI - 10.3402/jev.v4.28094
Subject(s) - microvesicles , extracellular vesicles , extracellular vesicle , biogenesis , characterization (materials science) , vesicle , library science , medicine , biology , nanotechnology , computer science , microbiology and biotechnology , genetics , microrna , gene , materials science , membrane
The UK–Russia extracellular vesicles (EVs) workshop was held at the Medical Center of the M.V. Lomonosov Moscow State University, Moscow, Russia, with 56 attendees from UK and Russian universities and research institutes. The program consisted of 6 research sessions and was focused on studies of EVs isolated from in vitro model systems or biological fluids, including blood and urine. The multidisciplinary program included presentations on mechanisms of EV biogenesis, the role of EVs in disease pathogenesis, the diagnostic value of EVs, including their quantitation and cargo load, as well as the clinical use of EVs in regenerative medicine. Methodological challenges imposed by the nanoscale size of EVs as well as targeted delivery approaches for therapeutics were considered in a separate session on technologies. The main aim of the workshop was to overview challenges confronting EV researchers and to facilitate knowledge exchange between researchers with different backgrounds and skills. Given the lack of definitive EV nomenclature, specific terms (exosomes or microvesicles) were only applied in the meeting report to studies that carried out full EV characterization, including differential ultracentrifugation isolation approaches, comprehensive protein marker characterization, and single vesicle analysis (electron microscopy and nanoparticle analysis), to ascertain EV size and morphology following the International Society for Extracellular Vesicles standardization recommendations (1,2). In studies where characterization was not conclusive, the term EV is used.