Open AccessPotentials and capabilities of the Extracellular Vesicle (EV) Array
Author(s) -
Jørgensen Malene Møller,
Bæk Rikke,
Varming Kim
Publication year - 2015
Publication title -
journal of extracellular vesicles
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 5.94
H-Index - 68
ISSN - 2001-3078
DOI - 10.3402/jev.v4.26048
Subject(s) - extracellular vesicles , microvesicles , extracellular vesicle , vesicle , phenotype , computational biology , chemistry , exosome , microbiology and biotechnology , nanoparticle tracking analysis , nanotechnology , biology , microrna , biochemistry , materials science , gene , membrane
Extracellular vesicles (EVs) and exosomes are difficult to enrich or purify from biofluids, hence quantification and phenotyping of these are tedious and inaccurate. The multiplexed, highly sensitive and high‐throughput platform of the EV Array presented by Jørgensen et al., (J Extracell Vesicles, 2013; 2: 10) has been refined regarding the capabilities of the method for characterization and molecular profiling of EV surface markers. Here, we present an extended microarray platform to detect and phenotype plasma‐derived EVs (optimized for exosomes) for up to 60 antigens without any enrichment or purification prior to analysis.